Mistic: Cellular localization, solution behavior, polymerization, and fibril formation

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FORTHERECORD Mistic: Cellular localization, solution behavior, polymerization, and fibril formation

Mistic represents a family of unique membrane-associating proteins originally found in Bacillus subtilis (M110). As a fusion partner, it has been shown to assist overexpression of foreign integral membrane proteins in E. coli. We have expressed shorter Mistic homologs from other Bacillus species and surprisingly, unlike M110, found them abundant in the cytoplasm. These Mistic homologs including...

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Mistic: cellular localization, solution behavior, polymerization, and fibril formation.

Mistic represents a family of unique membrane-associating proteins originally found in Bacillus subtilis (M110). As a fusion partner, it has been shown to assist overexpression of foreign integral membrane proteins in E. coli. We have expressed shorter Mistic homologs from other Bacillus species and surprisingly, unlike M110, found them abundant in the cytoplasm. These Mistic homologs including...

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Localization microscopy for the study of amyloid fibril formation

Super-resolution microscopy has emerged as a powerful and non-invasive tool for the study of molecular processes both in vitro, but also as they occur in live cells. Here we present the application of direct stochastic optical reconstruction microscopy (dSTORM), a super-resolution technique based on single molecule localization, to determine the morphology of protein aggregates and of small ext...

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ژورنال

عنوان ژورنال: Protein Science

سال: 2009

ISSN: 0961-8368

DOI: 10.1002/pro.148